A1,
A2,
B,
C1,
C2,
D,
E,
F,
G-H,
I-K,
L,
M,
N,
O,
P1,
P2,
Q-R,
S,
T,
U-V,
W-Z
Infergen Online, Description, Chemistry, Ingredients - Interferon alfacon-1
Infergen Online, Description, Chemistry, Ingredients - Interferon alfacon-1
DESCRIPTION
Interferon alfacon-1 is a recombinant
non-naturally occurring type-I interferon. The 166-amino acid
sequence of Interferon
alfacon-1 was derived by scanning
the sequences of several natural
interferon alpha
subtypes and assigning the most frequently observed amino acid
in each corresponding position.1 Four additional amino
acid changes were made
to facilitate the molecular construction, and a corresponding
synthetic DNA
sequence was constructed using chemical
synthesis methodology.
Interferon alfacon-1 differs from interferon
alfa-2 at 20/166 amino acids (88% homology), and comparison with
interferon-beta shows identity
at over 30% of the amino acid positions, a greater similarity than
any natural interferon
alpha subtype. Interferon alfacon-1 is produced in Escherichia
coli (E coli) cells that have been genetically altered
by insertion of a
synthetically constructed sequence
that codes for Interferon alfacon-1. Prior to final purification,
Interferon alfacon-1 is allowed to oxidize
to its native state, and its final purity
is achieved by sequential
passage over a series
of chromatography
columns. This protein
has a molecular weight
of 19,434 daltons. Infergen® is the Amgen Inc. trademark for
Interferon alfacon-1.
Infergen is a sterile,
clear, colorless, preservative-free liquid
formulated with 100 mM sodium
chloride and 25 mM
sodium phosphate
at pH 7.0±0.2. The
product is available
in single-use vials containing 9 mcg
and 15 mcg Interferon alfacon-1 at a fill volume
of 0.3 mL and 0.5 mL, respectively. Infergen vials contain 0.03
mg/mL of Interferon alfacon-1, 5.9 mg/mL sodium chloride, and 3.8
mg/mL sodium phosphate
in Water for Injection, USP. Infergen is to be administered undiluted
by subcutaneous
(SC) injection.
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